动物造模,凝血酶法致大鼠血液高凝态模型 z-bio 2022-11-22 223

　　Objective: To explore the rat model of hypercoagulable state induced by intravenous thrombin, and to provide a suitable animal model for the study of hypercoagulable state.

　　Methods: SD rats were divided into six groups. Normal saline and 2.5, 5, 10, 20, 40 U/kg thrombin solution were injected into femoral vein at constant speed. Blood samples were collected within 5 minutes to measure activated partial thromboplastin time, prothrombin time, fibrinogen, and observe the death of rats to determine the optimal dosage of thrombin. On this basis, the rats were injected with the optimal dose of thrombin solution through femoral vein, and the blood samples were collected at 0, 10, 30, 60, 120, 180, 300 s respectively for APTT, PT, FIB to determine the optimal blood collection time. Finally, the rats were divided into normal saline group and thrombin group (optimal thrombin dosage and blood collection time), and blood samples were collected to measure APTT, PT, FIB and whole blood viscosity.

　　Results: In the 10 U/kg thrombin group, the plasma APTT and PT were significantly shortened, FIB was significantly increased, and the mortality was low. The plasma APTT and PT of rats were shortened the most and FIB content increased to the highest 60 s after thrombin injection. Compared with normal saline group, PT and APTT in plasma of rats in thrombin group were significantly shortened, FIB and whole blood viscosity were significantly increased, and the difference was significant (P<0.05).

　　Conclusion: The rat blood hypercoagulable model can be reproduced by injecting thrombin solution. The optimal dose of thrombin is 10 U/kg, the concentration of thrombin is 2 U/mL, and the optimal time for blood collection and test is 60 s.