动物造模,房颤模型 z-bio 2022-11-22 279

　　Objective: To improve the traditional method of stellate ganglion exposure in dogs, establish a new method of stellate ganglion exposure in dogs, and improve the success rate of modeling sympathetic nerve related atrial fibrillation.

　　Methods: Twenty eight adult dogs were randomly divided into the traditional group and the modified group, 14 in each group. Sympathetic associated atrial fibrillation model was established in 28 dogs. During the operation, the vital signs and survival rate of dogs were recorded, the voltage required to stimulate the stellate ganglion was recorded, and the changes of cardiac electrophysiology before and after stimulation were recorded. The release of neurotransmitters was detected by immunohistochemistry.

　　Results: The intraoperative survival rate of the improved group was significantly higher than that of the traditional group (P<0.05). The operation time of the improved group was significantly shorter than that of the traditional group (P<0.05). The voltage required to stimulate the nerve in the improved group to reach the threshold was smaller than that in the traditional group (P<0.05).

　　Conclusion: The improved operation method can effectively reduce the mortality of dogs during the operation, significantly shorten the operation time, reduce the amount of bleeding during the operation, the stellate ganglion structure is more complete, and the voltage required during continuous stimulation is more stable, which is more suitable for establishing sympathetic related atrial fibrillation model.