动物实验,糖尿病 z-bo 2020-08-22 584

　　Objective: To study the effect of Tripterygium wilfordii polyglycoside on the expression of hypoxia-inducible factor-1α (HIF-1α) and endothelin-1 (ET-1) in the kidney tissue of diabetic rats, and to study its damage to the kidneys The protective effect and possible mechanism of vine polyglycoside on tomato.

　　Methods: Using streptozotocin (55 mg/kg body weight), normal control group, diabetic control group and pancreatic p-polysaccharide low, high-dose treatment group (8, 16 mg/kg) induced diabetic rats were divided into) and positive Control group (Irbesartan 50mg/kg), once a day, administered in the stomach, fasting blood glucose (BG), blood creatinine (Scr), urea nitrogen (BUN), 24-hour urine protein (24hUpro) every 8 weeks . HE staining optical microscope for morphological changes of kidney tissue, pathological image analysis system for analyzing mean glomerular area (MGA) and mean glomerular volume (MGV), immunohistochemistry and protein detection of HIF in kidney tissue Blot 1α and ET-1 protein expression; real-time fluorescent quantitative PCR to detect HIF-1α and ET-1 mRNA expression in kidney tissue.

　　Result: The expression of HIF-1α and ET-1 in the kidney tissue of diabetic rats increased. Compared with the diabetic control group, the Scr, BUN, 24hUpro, renal mass index (KW/BW), MGA and MGV of rats in each treatment group were significantly reduced, and the pathological morphology of the kidney tissue was significantly improved. HIF-1α, ET-1 mRNA and protein expression were significantly reduced; all indexes of the high-dose Tripterygium wilfordii polyglycoside I polyglycoside group were significantly improved. Correlation analysis showed that the expression of ET-1 was positively correlated with the expression of HIF-1α, while the expression of mRNA and protein of HIF-1α and ET-1 was determined by renal mass index (KW/BW), 24hUpro, and MGA. , Has a significant positive correlation with MGV.

　　Conclusion: The expression of HIF-1α and ET-1 in the kidney tissue of diabetic rats increases. Tripterygium wilfordii polyglycosides can improve the kidney damage of diabetic rats by inhibiting the expression of HIF-1α and ET-1, and delay the onset and onset of diabetic nephropathy.