动物造模,阿尔茨海默病树鼩模型 z-bio 2022-11-23 270

　　Objective: To analyze the brain imaging characteristics of tree shrews in Alzheimer disease model.

　　Methods: A was injected into the lateral ventricle of brain stereotaxic instrument β 1-40 Establish AD animal model. After the success of the model was confirmed by visual spatial behavior testing, MRI was used for coronal T2 weighted imaging (T2WI) and diffusion tensor imaging (DTI) analysis.

　　Results: The reference memory errors (3 weeks, 4 weeks) and working memory errors (2 weeks, 3 weeks, 4 weeks) in the model group were significantly higher than those in the control group (P<0.05). The time to complete the task in the model group (2 weeks, 3 weeks) was significantly longer than that in the control group (P<0.05). From 3 weeks on, the unilateral or bilateral hippocampus of tree shrews in the model group decreased, and the corresponding lateral or bilateral ventricles increased. At 12 weeks, the width of bilateral temporal angle in the model group was significantly greater than that in the control group and the treatment group (P<0.01). Diffusion tensor imaging scanning showed that the bilateral apparent diffusion coefficient (ACD) of hippocampus in the model group was greater than that in the control group (P<0.01). The loss of callosal fiber bundle was serious in model group.

　　Conclusion: Lateral ventricle injection A β 1-40 may cause learning and memory disorders in tree shrews. MRI can show the characteristic changes in the brain of AD tree shrews. The width of temporal angle, the ADC value of hippocampus, and the damage of corpus callosum fibers are of reference value in the diagnosis of dementia.