动物造模,可诱导性肝损伤模型 z-bio 2022-11-23 255

　　Objective: To establish an Alb cre/DTR double transgenic mouse model and analyze the related phenotypes. On this basis, an inducible liver damage model was established for the study of liver diseases.

　　Methods: The introduced Alb cre and DTR mice were propagated, and the double transgenic mice were obtained by hybridization. DNA was extracted from tail tissues of mice and genotyped by PCR. Diphtheria toxin was injected intraperitoneally into the double transgenic mice, and then weighed and blood was collected at different time points to detect the serum ALT and AST levels.

　　Results: Alb cre and DTR mice were hybridized and screened to obtain Alb cre/DTR double transgenic mice. The use of 0.625 ng/g diphtheria toxin in the mice could significantly increase the levels of ALT and AST in the serum of the mice. After dissecting the mice, it was observed that the whole liver turned white, and the HE staining results showed that the liver cells were obviously necrotic.

　　Conclusion: The mouse model of inducing specific liver injury was successfully established.