动物造模,脓毒症模型 z-bio 2022-11-23 264

　　Objective: To establish and evaluate the sepsis model in mice, so as to provide a model animal for studying the pathogenesis of sepsis and developing anti-inflammatory drugs.

　　Methods: Sepsis in mice was induced by cecal ligation and puncture, and the model was evaluated in terms of animal survival, post operation bacterial load, blood routine test, blood biochemical indexes, cytokine levels, and histopathological changes.

　　Results: The mortality of mice was closely related to the cecal ligation site. The 12 day survival rate of 50% mice with cecal ligation was about 40%, and the 4 day survival rate of 75% mice with cecal ligation was all dead (P<0.01). Compared with the sham operation group, the bacterial load in the blood and abdominal cavity of 50% CLP ligated mice increased, while the leukocytes decreased (P<0.001). The levels of ALT, AST in liver and BUN in serum of CLP mice increased significantly (P<0.01) α、 IL6、IL10、MIP1 α、 MIP1 β、 TNF α The level rises. The liver and lung of the mice showed obvious histopathological damage 48 hours after operation.

　　Conclusion: The mouse CLP model has typical pathological characteristics of sepsis, which provides a better animal model for the later study of anti inflammatory drugs screening.