动物造模,妊娠期糖尿病小鼠模型 z-bio 2022-11-24 307

　　Objective: To explore the method of establishing a model of insulin resistance in pregnancy.

　　Methods: 60 SPF 5-week old KM mice were randomly divided into a high-fat diet group and a general diet group. After four weeks of high-fat diet, the rats in the high-fat diet group were caged with 1:1 male and female, and the vaginal suppository was seen in the vagina of the female rats on the first day of pregnancy. After successful pregnancy, intraperitoneal injection was conducted at an interval of 30 mg/kg for 24 hours for three times, and the control group was injected with the same amount of citric acid buffer (0.1 mol/L, pH=4.2). On the 3rd, 7th, 14th and 19th days after the successful establishment of the model, the random blood glucose, body weight, water consumption and food intake in 24 hours of the mice were recorded. The concentrations of INS, ADP, LEP and CRP factors in serum were detected by ELISA.

　　Results: After the model was successfully established, the pregnant rats showed obvious symptoms of increased water consumption, food intake and urine output. The water consumption and food intake were significantly different from those in the control group (P<0.01). the="" blood="" glucose="" in="" model="" group="" was="">11.1 mmol/L, significantly higher than that in the control group. In GDM group, INS (1.50 ± 0.25) Mu/L, ADP (0.65 ± 0.13) μ g/L,LEP(1.60±0.12) μ g/L,CRP(37.54±4.70) μ G/L, compared with the control group, the difference was very significant (P<0.01). After delivery, the blood sugar of mice returned to normal level.

　　Conclusion: The GDM model can be established by high-fat diet combined with multiple induction of low-dose STZ, which is consistent with the characteristics of pathological insulin resistance in human gestational diabetes.