动物造模,大肠埃希菌致兔慢性输卵管炎症模型 z-bio 2022-11-24 287

　　Objective to establish a new zealand rabbit model of chronic oviduct inflammation using escherichia coli.

　　Methods Prepare the mixed bacterial solution (Escherichia coli: Staphylococcus aureus: Streptococcus) and 3 × 108/mL Escherichia coli suspension. Eighteen New Zealand female rabbits were randomly divided into three groups, including 2 in the normal group, 8 in the mixed bacterial solution group and 8 in the Escherichia coli solution group. The mixed bacterial solution and Escherichia coli solution were respectively injected into the rabbits of the two groups through vaginal intrauterine intubation and perfusion. The gross specimens and the formation of salpingitis under light microscope were observed at 5,10,15,20 days after intrauterine instillation of bacterial fluid.

　　Results The rabbits in the mixed bacterial solution group and the Escherichia coli solution group showed chronic salpingitis on the 15th day of the perfusion operation.

　　Conclusion The two modeling methods have the advantages of close to human infection route, high modeling rate and simple operation, and are effective methods for establishing animal models of chronic fallopian tube inflammation.