动物实验,丹参酮IIA 磺酸钠 z-bo 2020-08-22 582

　　Objective: To evaluate the preventive and therapeutic effects of tanshinone IIA sulfonate (STS) on postoperative peritoneal adhesions in experimental rats, and to explore its mechanism.

　　Method: establish a rat model of postoperative peritoneal adhesions. 75 rats were randomly divided into model group, STS high-dose group, middle-dose group, low-dose group and blank group. Each group had 15 rats, 20 and 10 STS high, medium and low groups respectively. And 5 mg/kg STS. The model control group was given the same amount of normal saline and injected intraperitoneally for 7 days. After 7 days, each group of rats was sacrificed to assess the adhesion level, the level of tPA/PAI-1 in the tissue was detected by ELISA, the activity of tPA in the peritoneal fluid was detected by the chromogenic substrate method, and the peritoneal tissue was detected. TGF is detected. Semi-quantitatively detected -B1 and Collgen-I in the IHC formula of the wound healing strength test to study whether STS affects wound healing.

　　Results: Compared with the model group, the STS high, medium and low dose groups reduced the occurrence of peritoneal adhesions; peritoneal fluid tPA activity increased significantly, and tissue tPA/PAI-1 protein significantly increased. Increasing it significantly increases its level, and significantly reduces TGF-β1 and Collgen-I protein expression. At the same time, intraperitoneal injection of STS will not affect wound healing.

　　Conclusion: Intraperitoneal injection of STS can effectively prevent the occurrence of postoperative adhesions. Its mechanism of action is to reduce the expression of TGF-β1 and down-regulate the activation and downstream of the TGF-β/Smads pathway. This may be due to the peritoneum caused by the decrease in PAI-1 level. Fibrinolysis is upregulated.