动物造模,异动症模型 z-bio 2022-11-24 291

　　Objective: To observe the effect of Shouwu Formula on behavioral indexes and amino acid neurotransmitters in the brain of rats with levodopa induced dyskinesia, and to explore the mechanism of Shouwu Formula in the intervention of dyskinesia.

　　Methods: The rat model of hemiparkinsonism was established by stereotactic injection of 6-hydroxydopamine into the brain. The model animals were given levodopa or L-DOPA+different doses of SWF by gavage. They were divided into L-DOPA group, L-DOPA+SWF low dose group, LID+SWF high dose group, and sham operation group. All animals were administered for 22 consecutive days. During the administration, the rats were scored for abnormal involuntary movement. On the 22nd day, microdialysis technology was used to sample the extracellular fluid of striatum of conscious rats, and high performance liquid chromatography fluorescence method was used to detect glutamate γ- The level of aminobutyric acid changes dynamically.

　　Results: After L-DOPA administration, AIM gradually appeared in rats, and at the end of administration, AIM score in L-DOPA group was significantly higher than that in sham operation group (P<0.01); Compared with the L-DOPA group, the AIM score of the L-DOPA+SWF high-dose group decreased significantly (P<0.05). On the 22nd day, compared with the sham operation group, the levels of Glu and GABA in the extracellular fluid of striatum of rats in each treatment group increased, and the levels of Glu and GABA in L-DOPA group showed statistical difference (P<0.05); Compared with the L-DOPA group, the Glu level in the high-dose L-DOPA+SWF group was significantly lower (P<0.05). 60 min after administration, the levels of Glu and GABA in L-DOPA+Shouwu Formula groups were significantly lower than those in L-DOPA group (P<0.05, P<0.01).

　　Conclusion: SWF can alleviate the side effects caused by L-DOPA and improve the behavioral symptoms of LID rats. Its mechanism may be related to the improvement of the abnormal levels of amino acid neurotransmitters in the brain of LID rats.