动物实验,肿瘤 z-bo 2020-08-22 445

      Objective: To study the effects of different doses of propofol on the expression of MTA1 and Wnt1 in the lung metastasis of rat MADB106 cells and tumor tissues.

　　Methods: Forty Fischer 344 male rats were randomly divided into the following groups: saline group (S group), fat emulsion group (F group), propofol 30 mg/kg group (P30 group) and 50 mg/kg kg group (P50 Group), 10 per group. Inject 1% sodium pentobarbital 50 mg/kg intraperitoneally into the femoral vein, and inject the same amount of the above-mentioned drugs, and 1 hour later, intravenously inject 0.5 ml of MADB106 tumor cells (2×105 cells). They were sacrificed after 3 weeks, and the number of lung metastases was counted; the expression of MTA1 and Wnt1 in lung tumor tissues was detected by immunohistochemistry, and quantitative analysis was performed by IPP software.

　　Results: There were no significant differences in the number of lung metastases and the expression of MTA1 and Wnt1 in tumor tissues between F group and S group (P\→0.05). Compared with the S group, the number of lung metastases and the expression of MTA1 and Wnt1 in the tumor tissues of the P30 and P50 groups were significantly reduced (P\u003c0.01). The number of lung metastases and the expression of MTA1 and Wnt1 were all negative at the dose of propofol. The correlation and Pearson correlation coefficients are -0.879, -0.80 and -0.916 respectively (P\u003c0.01). The expression of MTA1 and Wnt1 was positively correlated, and the Pearson correlation coefficient was 0.902 (P\u003c0.01).

　　Conclusion: Propofol dose-dependently inhibits the lung metastasis of MADB106 tumor cells, and down-regulates the expression of MTA1 and Wnt1 in metastatic tumors.