动物造模,缺血心肌 z-bio 2022-12-01 332

　　Objective To explore the protective mechanism of Nishixionghuang Powder on ischemic myocardium in mice.

　　Methods C57BL/6J mice were randomly divided into blank group (Blank), sham operation group (sham), model group (AMI), realgar group (XH), saltpeter group (XS) and saltpeter realgar group (XSXH). The levels of serum cardiac troponin I (cTnI), creatine kinase isoenzymes (CK-MB) and lactate dehydrogenase (LDH) were detected by ELISA at 12,24 and 36 hours after the model establishment; On the 14th day after modeling, the content of total NO in serum of each group was detected with nitric oxide (NO) kit; The pathological damage of infarcted myocardium in mice was detected by hematoxylin eosin staining, and the infarcted area was determined by 2,3,5-triphenyltetrazolium chloride staining; At 12h and 14d after taking realgar and nitrate realgar powder, liver and kidney function indexes such as glutamic pyruvic transaminase, alkaline phosphatase, glutamic oxaloacetic transaminase, creatinine and urea nitrogen in serum of each group were detected with relevant kits.

　　Results The levels of cTnI, CK-MB and LDH in serum of mice in the nitrate realgar group were significantly lower than those in the model group, the realgar group and the nitrate group (P<0.05); The level of NO in serum of sham operation group was higher, and the level of NO decreased significantly after myocardial ischemia (P<0.05); Compared with the model group, realgar group and nitrate group could significantly increase the NO level in mice with myocardial infarction (P<0.01); Compared with single saltpeter or realgar, the content of NO in saltpeter realgar group was significantly higher (P<0.001); Compared with the model group, the myocardial infarction area of mice in the nitrate realgar group was significantly reduced (P<0.05); This prescription can obviously improve the pathological damage of myocardium in mice. Compared with the blank group, the indexes of liver and kidney function in realgar group were significantly increased (P<0. p="">0. 05).

　　Conclusion When the LArgine-eNOS-NO pathway is inhibited during myocardial ischemia, Nishixionghuang Powder can regulate the NO level in mice through the NO3 -- NO2 -- NO pathway, and play a significant protective role in the ischemic myocardium of mice; It has no obvious acute and subacute toxic effects on the liver and kidney functions of mice.