动物造模,抑郁大鼠 z-bio 2022-12-02 277

　　Objective To establish a depression model of SD rats by chronic mild unpredictable stimulation, and to explore the effects and mechanisms of different styles of music in improving depression like behavior in SD rats.

　　Methods CUMS rats were constructed by chronic unpredictable stimulation lasting for 4 weeks after orphanage. Thirty nine CUMS depression rats were selected by sugar preference test and forced swimming test. They were randomly divided into model group, light music group and classical music group, and another control group without any stimulation was set as a blank control. The light music group and the classical music group respectively played Kushirang music and Mozart music for 4h every day, and carried out SPT, FST and O maze test (0-maze) immediately after 3 weeks. Take materials immediately after the test. HE staining was used to observe the structural changes of hippocampus, immunohistochemistry and Westem Blot were used to detect the expression level of brain-derived neurotrophic factor, and liquid chromatography tandem mass spectrometry was used to detect the content of corticosterone pentahydroxy in hippocampus. Detection of BDNF, CORT, 5-HT and IL-1 in peripheral blood by ELISA β Concentration change of.

　　Results Compared with the model group, the sugar preference percentage of the rats in the light music group and the classical music group increased (P<0.05), the FST immobility time decreased (P<0.01), and the times of entering the open arm of the O maze and the retention time increased (P<0.05). Compared with the model group, the hippocampal structure of the light music group and the classical music group was complete and orderly arranged, and the expression level of BDNF was increased (P<0.01). After music intervention, the serum BDNF level was significantly increased in both groups (P<0.01). The content of CORT in hippocampus of two music groups decreased (P<0.01), and the content of CORT in serum of classical music group decreased significantly (P<0.01). Serum 5-HT content increased in light music group (P<0.01), but there was no significant difference in classical music group. Serum IL-1 in two music groups β The concentrations were significantly decreased (P<0.01).

　　Conclusion Both classical music and light music can improve the behavior of depression model rats to some extent.