动物实验,急性肺损伤 z-bo 2020-08-22 542

　　Objective: To explore the protective effect of Ulinastatin (UTI) on acute lung injury in mice with severe heatstroke.

　　Method: 60 SPF male C57/BL6 mice were randomly divided into 6 groups (n = 10): normal group, severe heatstroke model group, treatment group, normal saline. Each group simulates the post-administration simulation group and the physiological saline group. The mice in the normal group were placed in an environment of (22±1)°C, and the mice in the other group were placed in a heating room (temperature 37°C, humidity 60%) and heated continuously. Before and after modeling, the two dose groups were injected intraperitoneally with UTI5×104U/kg for 10 minutes, and the same volume of normal saline was injected intraperitoneally into the two saline groups. .. Manually measure the rectal temperature (Tc) with a mercury thermometer every 30 minutes for 1.5 hours, and then every 15 minutes before heating. After the second rectal temperature, when Tc reaches 42.7°C (indicating severe heat stroke), leave the hot room and allow it to reach room temperature for 6 hours. Pathological changes of each group of lung tissue, protein content of bronchoalveolar lavage fluid (BALF), lung dry/wet ratio (W/D), lung water content and detected pulmonary vascular permeability (observe Evans Blue). content).

　　Results: The severe heat stroke model group showed obvious pathological changes before and after modeling and the normal saline group, lung W/D ratio, lung hydration injury, BALF volume, protein content and obvious damage to lungs and blood vessels. The permeability increased significantly (P0.05). Compared with the severe heatstroke model group and the normal saline group before and after the model treatment, the lung pathological changes in the treatment group were significantly improved. The lung W/D value, lung water content, and BALF protein content in the pretreatment group, Tc≥42.7°C Higher than other heat shocks, but higher than the normal control group (all P≥0.05). It is that although the group was significantly prolonged, the aforementioned lung injury index did not improve significantly.

　　Conclusion: Unastatin has an inhibitory effect on pulmonary edema and inflammatory exudate caused by severe heat stroke, and can effectively resist and prevent acute lung injury caused by severe heat stroke.