动物造模,心脏缺血再灌注损伤 z-bio 2022-12-06 371

　　Objective To investigate the effect of fentanyl combined with propofol on myocardial ischemia-reperfusion injury in rats based on autophagy.

　　Methods Thirty healthy male SPF SD rats were randomly divided into 5 groups: sham operation group, model group, fentanyl treatment group, propofol treatment group and fentanyl+propofol treatment group. Except the sham operation group, the rest of the rats established myocardial ischemia-reperfusion injury models. The fentanyl treatment group, the propofol treatment group and the fentanyl+propofol treatment group were respectively used with 20 μ G/kg fentanyl, 50 mg/kg propofol and 20 μ G/kg fentanyl combined with 50 mg/kg propofol pretreatment. The rats were killed 30 minutes after the end of the experiment, and the cardiac tissues were taken for subsequent detection. The pathological damage of rat heart tissue was observed by HE and TUNEL staining, the myocardial infarction area was observed by TTC staining, the formation of myocardial autophagic vesicles was observed by electron microscopy, and the expression of autophagy related proteins Beclin1, ATG5 and LC3B was detected by Western blot.

　　Results Compared with the sham operation group, the pathological changes of myocardium in the model group were more severe, the percentage of apoptosis and infarcted area of cardiac cells increased, the mitochondria of cardiac cells swelled, and the expressions of autophagy related proteins Beclin1, ATG5, and LC3B-II increased; Compared with the model group, 20 μ G/kg fentanyl, 50 mg/kg propofol and 20 μ Pretreatment with g/kg fentanyl combined with 50 mg/kg propofol significantly reduced the myocardial pathological damage of I/R rats, decreased the percentage of cardiac cell apoptosis and infarct area, reduced the degree of myocardial cell mitochondrial swelling, and reduced the expression of autophagy related proteins Beclin1, ATG5, and LC3B-II μ The pretreatment of g/kg fentanyl combined with 50 mg/kg propofol performed best, but there was no significant difference between fentanyl and propofol.

　　Conclusion Both fentanyl and propofol can inhibit autophagy and mitochondrial swelling of myocardial cells, and reduce myocardial I/R injury μ There is no significant difference between g/kg fentanyl and 50 mg/kg propofol in the treatment of myocardial I/R injury. In addition, the combination of fentanyl and propofol has synergistic effect on myocardial I/R injury