动物造模,急性胰腺炎大鼠肝损伤 z-bio 2022-12-06 312

　　Objective To investigate the protective effect of Dahuang Mudan decoction on liver injury in rats with acute pancreatitis.

　　Methods Ninety six SPF Wistar rats were randomly divided into blank group, model group, high, middle and low dose groups of Dahuang Mudan decoction (14, 7, 3.5 g/(kg · d)) and octreotide group (10 μ G/(kg · d. Observe the general signs of rats, biochemical determination of serum amylase (AMS), alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholinesterase (ChE), C-reactive protein (CRP) content, HE staining to observe the pathological changes of pancreas and liver tissue, ELISA to detect TNF in liver tissue of rats- α、 IL-1 β、 IL-6 content; Western Blot Assay of PI3K/Akt/NF in Rat Liver- κ The expression level of key proteins of B pathway.

　　Results (1) Compared with the blank group, the general survival condition of the model group rats was relatively poor, the contents of AMS, ALT, AST, CRP increased, and the content of ChE decreased; Under HE staining microscope, pancreatic tissue structure was incomplete, necrosis, hyperemia and inflammatory infiltration were serious, and liver cells were in disorder, necrosis, lipoid degeneration and inflammatory infiltration were obvious; TNF in liver homogenate- α、 IL-1 β、 The content of IL-6 increased significantly; PI3K, p-Akt, p-NF in liver tissue- κ The expression level of Bp65 and Bax protein increased, but the expression level of Bcl-2 protein decreased (P<0.05). (2) Compared with the model group, the survival status of rats in each treatment group was improved; Microscopically, the degree of edema, congestion and necrosis of pancreatic tissue was significantly improved, and the disorder, necrosis, lipoid degeneration and inflammatory infiltration of liver cells were significantly reduced; The contents of AMS, ALT, AST and CRP decreased in varying degrees, while the content of ChE increased; TNF in liver homogenate- α、 IL-1 β、 The content of IL-6 decreased; PI3K, p-Akt, p-NF in liver tissue- κ The expression level of Bp65 and Bax protein decreased, and the expression level of Bcl-2 protein increased (P<0.05).

　　Conclusion Dahuang Mudan Decoction can effectively improve the general state, biochemical indexes and pathological changes of pancreas and liver tissues in rats with acute pancreatitis, and its mechanism may be through regulating PI3K/Akt/NF- κ B signal pathway, thereby reducing liver injury.