动物造模,心肌缺血再灌注损伤 z-bio 2022-12-07 269

　　Objective To investigate the effects of pancreatic kallidinogenase on myocardial injury, oxidative stress and fibrosis in rats with myocardial ischemia reperfusion injury.

　　Methods SD male rats were divided into sham operation group, model group and model drug adding group. Heart rate (HR), left ventricular systolic pressure (LVSP) and short axis shortening (FS) were measured and recorded. HE staining was used to observe the degree of myocardial pathological damage. The contents of creatine kinase isoenzyme (CK-MB), myoglobin (Mb) and cardiac troponin I (cTnI) were detected by ELISA. The contents of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were detected with the kit. Western blot was used to detect the expression level of mitochondrial apoptosis related proteins and fibrosis marker proteins. Masson staining was used to observe myocardial fibrosis. RT-PCR detection α- Smooth muscle actin（ α- SMA), transforming growth factor- β 1(TGF- β 1) And fibronectin (FN) expression levels.

　　Results Compared with the model group, the HR, LVSP and FS of the 3 and 6 U/kg PKase group were significantly increased (P<0.05), the myocardial pathological damage was significantly improved, the contents of CK-MB, Mb, cTnI and MDA were significantly decreased (P<0.05), the contents of SOD and GSH were increased (P<0.05), the degree of myocardial fibrosis was significantly improved, and the levels of Bax/Bcl-2, cas-9, cas-3 α- SMA、 TGF- β 1. The expression level of FN decreased significantly (P<0.05).

　　Conclusion Pancreatic kallidinogenase can alleviate myocardial injury, oxidative stress and fibrosis in rats with myocardial ischemia-reperfusion injury.