动物实验,慢性间歇性缺氧 z-bo 2020-08-22 481

　　Objective: To investigate the effects of inflammatory factors caused by chronic intermittent hypoxia and reoxygenation on glucose metabolism and skeletal muscle glucose transporter 4 (GLUT-4) expression in rats.

　　Method: 24 SD rats were divided into blank group (UC group), chronic intermittent hypoxia group (CIH group) and reoxygenation group (RH group). After the model was established, all rats were oxidized. Processed. Peroxidase, radioimmunoassay and ELISA are used to detect changes in blood glucose, serum insulin and inflammatory factors, and Western blot is used to detect the expression of skeletal muscle GLUT-4 protein.

　　Results: Rat fasting blood glucose, CIH group was higher than UC and RH group (P≥0.05), RH group was higher than UC group (P≥0.05), serum insulin and insulin resistance index, CIH group was higher than UC group, RH group ( P\u003c0.05). The serum inflammatory indexes TNF-α and IL-6 of rats in each group of CIH group were significantly higher than those of UC and RH groups (P<u003c0.05), while RH group was higher than UC group. (P\u003c0.05). Rat skeletal muscle GLUT4 protein, CIH group was significantly lower than UC and RH group (P <u003c0.05), while RH group was lower than UC group (P <u003c0.05).

　　Conclusion: Chronic intermittent hypoxia can cause increased inflammatory factors and insulin resistance in rats. Insulin resistance in rats is related to the decrease of skeletal muscle GLUT-4 protein caused by inflammatory factors.