动物造模 z-bio 2022-12-07 350

　　Objective To observe the effects of Ginkgo honey ring oral solution (Yinmi for short) on the proliferation, migration, cell tubule formation and the expression of VEGF (vascular endothelial growth factor) and CD31 (platelet endothelial cell adhesion molecule) proteins of CMECs (rat myocardial microvascular endothelial cells), and to explore the angiogenesis ability of Yinmi in vitro.

　　Methods CMECs were cultured and divided into control group, high (2.6mg/mL), medium (1.3mg/mL) and low (0.6mg/mL) dosage group of silver honey. The proliferative ability of CMECs was detected by CCK-8 method; The migration ability of CMECs was detected by cell scratch test; The ability of CMECs to form tubes was tested by Matrigel angiogenesis experiment; The expression of VEGF and CD31 protein was detected by Western blot.

　　Results Compared with the control group, high, medium and low doses of silver honey could significantly promote the proliferation of CMECs; And the cell proliferation rate of high silver honey group was significantly higher than that of medium and low silver honey groups. After 6 hours of scratches, the cell migration rate of high silver honey group was significantly higher than that of the control group, while the cell migration rate of medium and low silver honey groups was higher than that of the control group, but there was no statistical difference; The CMECs migration rate of high, medium and low group of silver honey increased significantly 24 hours after scratch, and the cell migration rate of high group of silver honey was higher than that of medium and low group of silver honey. The mesh number, mesh area and cross points of CMECs in the high silver honey group were significantly better than those in the control group, the middle and low silver honey groups. The expression of VEGF and CD31 protein in the high and middle group of Yinmi increased significantly.

　　Conclusion Ginkgo honey ring oral solution can promote the proliferation, migration and angiogenesis of CMECs in vitro, as well as the expression of VEGF and CD31 protein, and its efficacy increases with the increase of the dosage of silver honey, suggesting that Ginkgo honey ring oral solution has a significant ability to promote angiogenesis in vitro.