动物造模 z-bio 2022-12-08 264

　　Objective To investigate the effects of T1L and NBV reovirus on intestinal flora in mice.

　　Methods 25 mice were randomly divided into 5 groups (control group, NBV intranasal group, NBV intragastric group, T1L intranasal group, T1L intragastric group) with 5 mice in each group. The control group was given PBS by gavage, and the other groups were given 2 × The mice were infected with 107 PFU/mL virus titer. Seven days later, mouse feces were collected, and three samples with heavy feces weight were selected from five samples in each group. The fecal DNA was specifically amplified in V3+V4 variable region, and 16S rRNA sequencing was used to analyze the abundance, diversity and species composition structure of bacteria in mouse feces.

　　Results Compared with the control group, the abundance and diversity of intestinal microflora in T1L and NBV group decreased, especially in T1L nasal drip group (P<0.05); Compared with the NBV intragastric group, the abundance and diversity of bacteria in the NBV intranasal group increased significantly (P<0.05). At the level of phylum, the abundance of Firmicutes in T1L and NBV groups decreased significantly, and the abundance of Bacteroides in T1L and NBV nasal drip groups decreased significantly; At the genus level, the abundance of Romboutsia in T1L nasal drip group, T1L intragastric drip group and NBV intragastric drip group decreased significantly, while the abundance of Alistipes in T1L nasal drip group increased significantly (P<0.05).

　　Conclusion Mice infected with T1L and NBV can reduce the abundance and diversity of bacterial flora, which may destroy the balance of bacterial flora by reducing beneficial bacteria or increasing pathogenic bacteria. In addition, the infection mode of the virus is different, and the influence on the flora is also different.