动物造模,肝纤维化 z-bio 2022-12-08 375

　　Objective To explore the effect of Camellia trifoliata L κ B- α And its phosphorylation down regulates the signal pathway of nuclear transcription factor to alleviate carbon tetrachloride induced liver fibrosis in rats.

　　Methods CCl4 rat model of liver fibrosis was used. Serum alanine aminotransferase (ALT), hydroxyproline (HYP) and transforming growth factor (TGF) were detected by biochemistry and ELISA respectively β 1(TGF- β 1)、 α- Smooth muscle actin（ α- SMA) level, interleukin-6 (IL-6), tumor necrosis factor- α ( TNF- α), Detection of Toll like receptor 4 (TLR4) mRNA and NF in rat liver by fluorescent quantitative PCR- κ B p65 mRNA、NF- κ B inhibitor protein- α (I κ B- α) MRNA expression, Western blot analysis of TLR4 and NF in rat liver- κ B p65、p-I κ B- α Protein expression and HE staining were used to evaluate the pathological damage of liver tissue.

　　Results Compared with the model group, Camellia trifoliata could significantly inhibit ALT, HYP and TGF in rats with liver fibrosis- β 1、 α- SMA、IL-6、TNF- α Level, improve the pathological damage of rat liver tissue, and significantly inhibit TLR4 mRNA and NF in rats with liver fibrosis- κ B p65 mRNA expression, up-regulated I κ B- α MRNA expression, down regulating TLR4 and NF- κ B p65 protein expression, down regulating p-I κ B- α Protein expression.

　　Conclusion Camellia trifoliata L. decreased I κ B- α Phosphorylation, NF inhibition- κ The activation of B signal pathway can achieve the effect of anti liver chronic inflammatory damage.