动物实验,慢性肾功能衰竭（CRF） z-bo 2020-08-22 577

　　Objective: To investigate the effect and mechanism of Wnt7a-Akt/mTOR signaling pathway in improving renal failure nutritional capsules for skeletal muscle atrophy in rats with chronic renal failure (CRF).

　　Method: establish a CRF model by removing 5/6 of the kidney, and feed a 4% casein diet to create a CRF malnourished rat model. The rats were randomly divided into normal group, model group, herbal medicine group and prescription group. Histopathology is used to detect the cross section of TA and calculate the distribution of muscle fiber area. The isotope 14 + C-phenylalanine incorporation method was used to detect muscle protein synthesis. Western blotting was used to detect protein expression in the Wnt7a-Akt/mTOR signaling pathway.

　　Result: Shenshuai Nutrition Capsule increased the body weight and skeletal muscle mass of CRF rats. The morphological manifestation is an increased TA cross section, which promotes the increase of protein synthesis and protein expression in the Wnt7a-Akt/mTOR signaling pathway in skeletal muscle tissue. up.

　　Conclusion: Shenshuai Nutrition Capsule can effectively improve skeletal muscle atrophy in CRF model rats. Its mechanism is related to up-regulating the Wnt7a-Akt/mTOR signaling pathway of skeletal muscle and promoting skeletal muscle protein synthesis. It may be