动物造模,急性肺损伤 z-bio 2022-12-12 345

　　Objective To investigate the intervention effect of chitosan on acute lung injury induced by PM2.5 in mice.

　　Methods Forty four male C57BL/6J mice were randomly divided into four groups: control group, PM2.5 group, chitosan group and chitosan+PM2.5 group. Chitosan+PM2.5 and chitosan groups were given chitosan by gavage 2 weeks in advance, while the control group and PM2.5 group were given distilled water by gavage once a day. Two weeks later, PM2.5 was instilled into the trachea of the PM2.5 exposed group, and normal saline was instilled into the control group and chitosan group once a day for 7 days. The animals were killed 24 hours after the last exposure. HE staining was used to observe morphology, and the levels of total protein (TP) and lactate dehydrogenase (LDH) in liver malondialdehyde (MDA) and alveolar lavage fluid (BALF) were measured by spectrophotometry; Determination of Interleukin-1 in BALF and Serum by ELISA β (IL-1 β)、 Interleukin-8 (IL-8) and tumor necrosis factor- α (TNF- α) expression.

　　Results In PM2.5 group, the alveolar septa were significantly widened, and there were obvious lymphoid and plasma cell infiltration; Compared with PM2.5 group, the pulmonary septum in chitosan+PM2.5 group was significantly narrowed, and the infiltration of endolymphatic cells and plasma cells was significantly reduced. Compared with the control group, MDA, TP, LDH, TNF in PM2.5 group- α、 IL-1 β And IL-8 significantly increased (P<0.05); Compared with PM2.5 group, chitosan, MDA, TP, LDH and TNF were supplemented in advance- α、 IL-1 β And IL-8 significantly decreased (P<0.05).

　　Conclusion Taking a certain dose of chitosan in advance has a certain intervention effect on lung injury induced by PM2.5.