动物实验 z-bo 2020-08-22 491

　　OBJECTIVE: To investigate the effect and mechanism of changing the subconscious cortex (IL) telemetry EEG activity on drug-seeking behavior in morphine-dependent rats during detoxification.

　　Method: The SD rats were randomly divided into model group and control group, and stereotactic electrodes were embedded in the IL area. The rats in the model group were sequentially constructed into a morphine-dependent model and a regression model, and a wireless telemetry system was used to record IL during the regression period. Changes in electrical activity in the local brain.

　　Result: The white box retention time of rats in the model group was significantly longer than before morphine injection and 1-2 days after drug withdrawal. There was no significant difference compared with the control group before injection. Comparing the model group with the control group, 2 days after stopping the drug, the EEG β wave in the interleukin area increased significantly, while the δ wave decreased significantly. When I shuttled from the black box to the white box, the delta wave increased significantly, while the alpha and beta waves decreased significantly. It was calm for two days. When the model group stays in the white box, compared with the control group, the EEG of the IL area is significantly reduced. Compared with the evacuation period, β waves and θ waves are significantly reduced, and δ waves are significantly increased. When shuttled from the black box to the white box, there was no significant difference in IL EEG compared with the control group, but compared with the withdrawal period, the δ wave was significantly reduced, and the α and β waves were significantly increased.

　　Conclusion: The abnormal changes of brain electrical activity in IL area of morphine-dependent rats during sedation of morphine-dependent rats may affect the formation of drug-seeking motivation and inhibit the development and maintenance of drug-seeking behavior.