动物造模,巨细胞病毒 z-bio 2022-12-13 333

　　Objective To study the inhibitory effect of cytomegalovirus (CMV) on immune response of mice implanted with polymethyl methacrylate (PMMA).

　　Methods The model of CMV infection in mice was established by intraperitoneal inoculation of virus suspension. After 7 days of virus inoculation, PMMA was implanted subcutaneously in the back of mice; The percentage of monocytes/macrophages, CD4+T cells, CD8+T cells and regulatory T cells (Treg cells) in peripheral blood was detected by flow cytometry; Detection of tumor necrosis factor by enzyme-linked immunosorbent assay (ELISA)- α (TNF- α) And interleukin-1 β (IL-1 β) Content of.

　　Results Compared with the control group, the proportion of MI monocytes/macrophages in the peripheral blood of CMV implanted mice decreased by 73.18% (=5.896, P=0.004), the proportion of M2 monocytes/macrophages increased by 2.18 times (t=7.971, P=0.001), the ratio of CD4/CD8T cells decreased by 42.91% (t=6.468, P=0.003), and the proportion of Treg cells increased by 2.49 times (t=4.495, P=0.011). Compared with the implanted control group, the peripheral blood TNF of mice implanted with CMV- α And IL-1 β The concentration of β - lactamase decreased by 71.65% and 57.95% respectively (t=7.236, P=0.019, t=7.543, P=0.002)

　　Conclusion CMV can significantly inhibit the immune response after PPMA implantation, which provides a theoretical basis for antiviral treatment of CMV infected patients after oral biomaterials implantation.