动物造模 z-bio 2022-12-14 291

　　Purpose discussion α- The effect of Roxniphyllin on the immune function of the immunosuppressive model of mice induced by cyclophosphamide provides an experimental basis for the follow-up mechanism research and the development of new drugs to increase immune function.

　　Methods The mice were randomly divided into 5 groups with 10 mice in each group: blank control group, immunosuppressive group α- High dose group, medium dose group and low dose group. In the first 7 days, except the blank control group, the other 4 groups were given cyclophosphamide (CTX) to establish the immunosuppressive model; After 14 days, the blank control group continued to be gavaged with normal saline, the immunosuppression group was gavaged with corn oil, and the three dose groups were gavaged with different doses of α- Reverse twist element. The mice were killed 24 hours after the last administration, and the delayed type hypersensitivity (DTH) test, serum hemolysin test (HC50), macrophage phagocytosis test (carbon clearance test), thymus and spleen index test of immune organs, natural killer cell (NK cell) activity test (MTT method), peripheral blood leukocyte count test, and spleen lymphocyte proliferation test were carried out α- Effect of Roxniphyllin on Immune Function in Mice.

　　Results The immunosuppressive model of mice was successfully established. Gavage α- After inversion, the three dose groups can improve the thymus and spleen index, half hemolysis value, spleen lymphocyte proliferation rate, NK cell activity of immunosuppressed mice in varying degrees, among which the high dose group, the total α- The stomach perfusion dose of Roxniphyllin was 100 mg/(kg · d), and its effect was the most outstanding. In many experiments, compared with the immunosuppressive group, the difference was very significant, with statistical significance (P<0.01); In delayed type hypersensitivity, there were significant differences between the high dose group, the blank control group and the immunosuppression group (P<0. p="">0. 05); In the peripheral blood leukocyte count test, there was a significant difference between the high-dose group and the immunosuppressive group (P<0. p="">0. 05); In the carbon clearance test, there was no significant difference between each dose group and the blank control group, immunosuppression group (P>0.05).

　　Conclusion Roxniphyllin can regulate the immune function of immunosuppressed mice, and the optimal dose of Roxniphyllin to improve the immune function of immunosuppressed mice is more in the high dose group, which is dose dependent in a certain range.