动物造模,慢性结肠炎模型 z-bio 2022-12-14 329

　　Objective To investigate the effect of Spirillum hepaticum infection on chronic colitis induced by dextran sodium sulfate in mice.

　　Methods Forty male IL-10 * mice were randomly divided into four groups: control group, H.h group, DSS group and H H+DSS group, H.h group and H In the h+DSS group, Hh was infected by gavage. After 5 days of HH infection, mice in DSS group and H, h+DSS group drank 2% DSS water continuously for 7 days, then returned to normal drinking water for 5 days, and then drank 2% DSS water continuously for 7 days; The rest groups drank water normally. After the experiment, the mice in each group were killed, the colon was taken and its length was measured, and then divided into three parts for histopathological examination, detection of cytokine mRNA transcription level and detection of protein expression level.

　　Results The results of pathological examination showed that compared with DSS group, the lymphocyte infiltration in H, h+DSS group was increased, the epithelial loss was serious, the degree of fibrosis was aggravated, the crypt atrophy was obvious, and the inflammatory cytokine interleukin 6 and tumor necrosis factor α The transcription level was up-regulated, and the expression level of signal transducer and activator of transcription 3 protein was increased.

　　Conclusion H.h infection can aggravate DSS induced colitis in mice.