动物造模 z-bio 2022-12-14 276

　　Objective To observe and analyze the effects of lipopolysaccharide (LPS) on the morphological structure and pathological indexes of lung tissue in nude mole rats, and to explore whether there is LPS tolerance in nude mole rats.

　　Methods Ten male naked mole rats were randomly divided into experimental group and control group. The experimental group was intraperitoneally injected with LPS at a dose of 10 mg/kg according to the body weight, and the control group was injected with 0.9% NaCI solution (normal saline) of the same volume. At 6h after administration, the lungs were collected and the wet to dry weight ratio of lung tissue was measured; Making paraffin

　　After HE staining, the microscopic structure of lung tissue was observed under microscope; The content of reactive oxygen species (ROS) in lung tissue cells and the apoptosis rate of lung tissue cells were detected by flow cytometry.

　　Results In the experimental group, the lung tissue structure of naked mole rats was intact, and there was no obvious exudate and inflammatory cell infiltration in the alveoli. Compared with the control group, the wet to dry mass ratio of lung tissue in the experimental group did not change significantly, and the early apoptosis rate did not change significantly, but the late apoptosis rate increased significantly (P<0.01), and the content of reactive oxygen species increased significantly (P<0.05).

　　Conclusion Nude mole rats have a certain degree of tolerance to LPS induced lung injury, and its specific mechanism needs further study.