动物造模,高原多脏器功能障碍综合征 z-bio 2022-12-14 278

　　Objective To study the tumor necrosis factor (TNF) in the high altitude multi organ dysfunction syndrome (MODS) model of pteridophyte pigs- α Interleukin-1 β And the change characteristics of C-reactive protein in the process of inflammatory reaction.

　　Methods Three doses (0.25, 0.35 and 0.50 mg/kg) of lipopolysaccharide (LPS) were used to induce plateau native Fern Ma pigs to successfully establish H-MODS models (groups B, C and D), and normal saline control group (group A) was set up at the same time. Detection of serum TNF at different time points- α、 IL-1 β And CRP expression level.

　　Results TNF of each group of pteridophyte pigs after pumping LPS- α、 IL-1 β The expression level increased sharply and reached the highest level at 6 hours, and increased significantly at 3-12 hours compared with the same group at 0 hours and at the same time point A (P ≤ 0.05 or P ≤ 0.01), and decreased gradually after 12 hours; Group B and D TNF- α The expression level in group B was significantly higher than that in group A at 0 h and the same time point after 24 h (P<0.05), and was significantly higher than that in group A at 72 h (P<0.05). 24 h IL-1 β The expression level in group D was higher than that in group 0 and group A (P<0.05), and then decreased gradually. At 48h and 72h, the expression level was significantly lower than that in group 0 and group A (P<0.05 or P<0.01). After LPS was pumped intravenously, the expression of CRP began to increase in groups B, C and D, and reached the maximum at 24-48 hours; Compared with group A and group 0, the expression of CRP in each group increased significantly at 6-72 h (P<0.05 or P<0.01).

　　Conclusion During the formation of H-MODS, the body produces serious inflammatory reaction, and the inflammatory factor TNF- α、 IL-1 β The expression level increased rapidly, but decreased rapidly under the effect of immune regulation, and fluctuated sharply in a short time to reach the equilibrium state; The change of CRP expression is relatively slow, accompanied by the whole process of inflammatory reaction in vivo.