动物造模,前列腺癌免疫人源化小鼠模型 z-bio 2022-12-15 374

　　Objective To construct a humanized mouse model of immune system and transplant it into a human prostate cancer cell line for the study of immunotherapeutic drugs for prostate cancer.

　　Methods Using Ficoll density gradient centrifugation, we separated the peripheral blood mononuclear cells (PBMC) from fresh human peripheral blood, and injected NPG mice through the tail vein to construct a mouse model with human immune system. At the 3rd, 4th, 5th and 6th weeks after injection, the peripheral blood of mice was collected by tail cutting method, and the dynamic changes of human CD45++CD3+T cells in the peripheral blood of mice were monitored by flow cytometry. The mice were given subcutaneous transplantation 3 weeks after injection × 10 'prostate cancer 2R1 cells were monitored twice a week for tumor growth. When the tumor grows to 1000 mm ² The mice were euthanized; Flow cytometry, immunohistochemistry and HE staining were used to analyze the infiltration of human immune cells in the peripheral blood, spleen and tumor tissues of humanized mice.

　　Results A high level of CD45+CD3+T cells was detected in the peripheral blood of the model mice 3~6 weeks after the transplantation of human PBMC. The mice were killed at the 6th week. The results of HE staining and immunohistochemical staining showed that there were human CD4+and CD8+T cells infiltrating the spleen and tumor tissues of the model mice.

　　Conclusion The mouse model of prostate cancer immune humanization has been successfully constructed, and there is a high level of human T cell infiltration in the peripheral blood, spleen and prostate cancer tumor tissue, which lays a foundation for the next step to build a good preclinical model of prostate cancer immunotherapy.