动物造模,肺癌异位皮下移植瘤 Z-bio 2022-12-19 374

　　Objective To investigate the effects of different concentrations of Matrigel on heterotopic subcutaneous transplantation of Lewis lung cancer in mice.

　　Methods Twenty four C57BL/6J Nifdc mice were randomly divided into lung cancer group, 50% matrix glue group, 75% matrix glue group and 100% matrix glue group, with 6 mice in each group. Lewis lung cancer cells (LL/2) were mixed with 0%, 50%, 75% and 100% Matrigel in a volume of 1 ∶ 1 respectively, subcutaneously injected under the armpit of the right forelimb of mice, and the weight, diet and water consumption of mice in each group were measured daily; The tumor formation time, tumor formation rate and tumor size were observed and compared. The long diameter and short diameter of the tumor were measured every day after the formation of the tumor, and the tumor volume was calculated; The animals were killed on the 15th day of tumor inoculation, the tumor tissues were completely stripped and weighed, and the pathological changes of tumor tissues were detected by HE staining.

　　Results Compared with the lung cancer group, the weight of mice in the 75% matrix glue group increased significantly from the second day of modeling (P<0.05 or P<0.01); The weight of mice in 100% matrix glue group increased significantly (P<0.05 or P<0.01) from the 10th day of modeling. The average diet of mice in each group decreased 8 days after the model was established, and the water consumption showed an overall upward trend. The tumor formation rate of mice in each group was 100%. The mice in the matrix glue group had earlier tumor formation time, faster tumor growth and larger tumor than those in the lung cancer group. Among them, the tumor volume in the 75% matrix glue group increased the fastest and the tumor weight was the largest (1358.88 ± 388.14) mg, which was significantly different from the tumor group (P<0.05); The tumor volume of the 100% matrix glue group increased next, and the tumor weight was (1142.37 ± 423.08) mg, which was significantly different from that of the tumor group (P<0.05); The tumor volume in the 50% matrix glue group grew the slowest and the tumor weight was the smallest, (808.83 ± 393.41) mg. The HE staining results showed that compared with the lung cancer group, the tumor cells in the matrix glue group grew vigorously, the outline was clear, the nuclear volume was larger, and the vascular components in the tumor tissue stroma were significantly increased.

　　Conclusion The matrix glue can stabilize the tumor formation rate, accelerate the tumor growth, and increase the tumor volume. The tumor growth is the fastest and the tumor volume is the largest under the 75% concentration of matrix glue, and the tumor homogeneity is good.