动物实验,亚急性吸入毒性 z-bo 2020-08-22 466

　　Purpose: To study the subacute inhalation toxicity of ivermectin to rats to achieve the maximum unobserved level of effect.

　　Method: Six SPF Sprague-Dawley (SD) rats were randomly divided into six groups. There are 12 rats in each group (half male and half male). Set up three dose groups, respectively 190, 380 and 750 mg/m3, as well as solvent control group (0.03% Tween-80 solution), blank control group and another dose group (750 mg/m3). The other 4 groups of animals were inhaled once a day, 4 days a week, 5 days a week, up to 28 days of inhalation of toxic substances, and observed for 14 days after stopping the poisoning. After the experiment, check the animal's blood, biochemistry, body weight, organ index, and conduct histopathological examination. Results The male and female rats in the 750 mg/m3 dose group showed toxic reactions during the later period of exposure, such as fluffy fur, dullness, salivation and tremor. In the 750 mg/m3 group, the food supply of female rats decreased, the serum alanine aminotransferase (ALT) level increased (P\u003c0.01) and the liver organ index (visceral ratio) increased (P\u003c0 ). .05) Histopathological examination showed that some rat liver cells were cloudy and swollen; male rats in the 750 mg/m3 dose group lost weight at 4 weeks and had serum urea. Nitrogen (BUN) and ALT levels increase (P \u003cu\→ c \u003c0.01), and total cholesterol (CHOL) decreases (P \u003cu\→ c0.05).

　　Conclusion: In the rat subacute inhalation toxicity test, the maximum unobserved level of ivermectin is 380 mg/m3 (4 h/d) for both males and females.