动物造模 Z-bio 2022-12-19 350

　　Objective To explore the regulation of melatonin on NF- κ The mechanism of B signal pathway regulating Th1/Th2 immune balance in gastric cancer mice.

　　Method will 50 μ G (approximately contains cell 1 × 106) gastric cancer cells were injected subcutaneously into the left forelimb of mice at one time, and tumor formation was felt 7 days after modeling, indicating that the modeling of gastric cancer transplanted tumor mouse model was successful. 10 healthy mice were used as the control group. The mice successfully modeled were divided into model group and model+melatonin group, with 10 mice in each group. In the model+melatonin group, melatonin was injected subcutaneously at multiple points (10 mg/kg, once a day, 21 days), and the other groups were injected with the same amount of normal saline. The tumor volume and weight of each group were measured with vernier caliper and balance. The proliferation and apoptosis of gastric cancer cells were evaluated by immunohistochemical detection of Ki67 and Bcl2 in tumor tissues. Detection of IFN in peripheral blood by ELISA- γ And IL-4. The proportion of Th1 and Th2 cells in peripheral blood was detected by flow cytometry. Detection of NF in tumor tissues and lymphocytes by qPCR and Western blot- κ B mRNA and protein levels.

　　Results Compared with the control group, the tumor volume, mass, Ki67 and Bcl2 staining intensity, IL-4 level, Th2 cell ratio, NF in model group and model+melatonin group- κ B mRNA and protein expression were significantly increased (P<0.05), IFN- γ Level, Th1 ratio and Th1/Th2 ratio decreased significantly (P<0.05); Compared with the model group, the tumor volume, mass, Ki67 and Bcl2 staining intensity, IL-4 level, Th2 cell ratio, NF in the model+melatonin group- κ B mRNA and protein expression decreased significantly (P<0.05), IFN- γ Level, Th1 ratio and Th1/Th2 ratio increased significantly (P<0.05). Conclusion Melatonin may regulate NF- κ B pathway inhibits tumor growth and regulates Th1/Th2 balance in gastric cancer transplanted tumor mouse model.