动物造模 Z-bio 2022-12-19 308

　　Objective To investigate the effect of reeling water on oxidative stress in aged rats.

　　Methods The process of traditional silk reeling water production was simulated, and the dry cocoon samples were processed, and the components of the silk reeling water were detected. The 16 month old rats were randomly divided into 3 test sample dose groups and 1 negative control group according to the content of malonaldehyde (MDA) in serum, with 10 rats in each group. The dose group was given 500, 250 and 125 mg/kg of reeling water respectively, and the negative control group was given pure water by gavage once a day for 30 consecutive days. After the experiment, the contents of lipid oxidation product MDA, protein oxidation product protein carbonyl, antioxidant reduced glutathione (GSH), and the activities of three antioxidant enzymes, namely superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH Px), were measured.

　　Results The crude protein content in reeling water was 1640 mg/100 g, the free amino acid content was 18 mg/100 g, and the hydrolyzed amino acid content was 1700 mg/100 g. Serine (30.59%), aspartic acid (18.82%), glycine (10.00%) and threonine (8.24%) are the main hydrolytic amino acids in reeling water. Compared with the negative control group, the reeling water can reduce the content of MDA and protein carbonyl in serum and tissues of aged rats (P<0.05), increase the content of GSH in serum and tissues (P<0.05), increase the activity of SOD in tissues (P<0.05), and increase the activities of CAT and GSH Px in serum and tissues (P<0.05).

　　Conclusion Silk reeling water can improve oxidative stress in aged rats.