动物造模,吸入综合征 Z-bio 2022-12-20 275

　　Objective To observe the protective effect of momordica grosvenorii saponin V on acute lung injury in rats with meconium aspiration syndrome (MAS) and explore its mechanism.

　　Methods Forty eight healthy Wistar rats were randomly divided into sham operation group, model group, low, middle and high dose of siraitin V group (2.5, 5, 10 mg/kg) and dexamethasone group (0.5 mg/kg), 8 rats/group; The MAS model was constructed by direct vision endotracheal intubation; The low, middle and high dose groups of siraitin V and the dexamethasone group were administered by gavage after modeling, and the model group and the sham operation group were administered by gavage with the same amount of normal saline. HE staining was used to observe the pathological changes of lung tissue; Measure the wet to dry mass ratio of lung tissue (W/D); Detection of tumor necrosis factor in serum and alveolar lavage fluid by ELISA α ( TNF- α)、 Interleukin 6 (IL - 10), Interleukin 1 β (IL-1 β) Horizontal; The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in lung tissue were detected by colorimetry; Western blot was used to detect the protein expression of c-Jun amino terminal kinase (JNK) and phosphorylated JNK (p-JNK) in lung tissue.

　　Results Compared with the sham operation group, the pathological score of lung tissue, W/D, alveolar lavage fluid and serum TNF in the model group increased (P<0.05)- α、 IL-6、IL-1 β The level of MDA and MPO in lung tissue increased, the level of SOD decreased (P<0.05), and the expression of p-JNK protein in lung tissue increased (P<0.05). Compared with the model group, the pathological score of lung tissue, W/D, and TNF in alveolar lavage fluid and serum in medium and high dose siraitin V groups and dexamethasone groups decreased (P<0.05)- α、 IL-6、IL-1 β The level of MDA and MPO in lung tissue decreased and the level of SOD increased (P<0.05); The expression level of p-JNK protein in lung tissue of middle and high dose siraitin V group was down regulated (P<0.05).

　　Conclusion Siraitia grosvenorii saponin V may play a protective role in acute lung injury by inhibiting JNK phosphorylation and alleviating inflammatory and oxidative stress injury in the course of MAS.