动物造模 Z-bio 2022-12-20 247

　　Objective To study the static inhalation toxicity of plastic runway surface materials in rats.

　　Methods The female and male SD rats that passed the quarantine inspection were randomly divided into four groups: the 30 day normal control group, the 30 day poisoned group, the 90 day normal control group and the 90 day poisoned group. There were 16 rats in each group, half male and half female, inhaled for 1 hour every day, and exposed for 30 and 90 days respectively. After the experiment, the rats in each group were dissected, the blood coagulation, blood routine, blood biochemical indexes were detected, the organ coefficients were calculated, and the histopathological examination was performed on each organ.

　　Results Compared with the normal control group in the same period, the thrombin time (TT) of male rats in the 30 day exposure group was shortened (P<0.05), and the activated partial thromboplastin time (APTT) of female rats in the 90 day exposure group was prolonged (P<0.01). After 30 days of exposure, serum Ca and P of female rats decreased (P<0.05, P<0.01), while serum creatinine (Cr) and urea nitrogen (BUN) of male rats increased; In the 90 day exposure group, serum BUN and P of female rats increased (P<0.05, P<0.01), Na and Cl decreased (P<0.01), and serum BUN and total protein (TP) of male rats increased (P<0.05). The adrenal gland coefficient of male rats in the 30 and 90 day exposure groups increased (P<0.01). In the 30 day exposure group, there were 3 cases of mild hepatocyte steatosis in the liver portal area, 3 cases of pulmonary small bronchial epithelial detachment, 1 case of renal cyst, and 1 case of testicular tubule atrophy. In the 90 day exposure group, there were 6 cases of mild hepatocyte steatosis in the liver portal area, 2 cases of pulmonary small bronchial epithelial detachment, 1 case of focal hypertrophy in the adrenal fascicular zone, 2 cases of atrophy of individual seminiferous tubules in the testis, 2 cases of renal glomerular telangiectasia, 1 case of renal cyst, 4 cases of clear tubular type, 1 case of mineralization, and 1 case of hydronephrosis.

　　Conclusion Under the experimental conditions, 30 days of inhalation exposure to the plastic runway surface material has less effect on the liver, lungs, testes and kidneys of SD rats, and 90 days of inhalation exposure has more severe damage on the kidneys of SD rats.