动物造模 Z-bio 2022-12-20 357

　　Objective To observe the intervention effect of resveratrol on premature ovarian failure in mice and elucidate its possible mechanism.

　　Methods Forty two month old female mice were randomly divided into four groups: control group, model group, 20 mg/kg RES group and 40 mg/kg RES group, with 10 mice in each group. The body weight, ovary weight and ovary/mouse relative weight ratio of mice were measured; HE staining was used to observe the number of follicles in mouse ovary; Detection of CyclinD1, Wnt1 and β- Catenin mRNA expression; Immunohistochemical staining in ovarian tissue β- Caterin protein expression; Western blot was used to detect the expression of Mvh, Oct4, SOD2, Nrf2, Bax and Bcl-2 proteins in ovarian tissue.

　　Results The body weight, ovary weight and ovary/mouse weight ratio of mice in 20 mg/kg RES group and 40 mg/kg RES group were higher than those in model group; Compared with the model group, the number of primordial follicles increased and the number of atresia follicles decreased in the 20 mg/kg RES group and 40 mg/kg RES group (P<0.05), while the number of cyclinD1, Wnt1 and β- Catenin mRNA expression increased (P<0.05); Compared with the model group, the ovarian tissue of mice in 20 mg/kg RES group and 40 mg/kg RES group β- The expression of catalin positive protein increased in a concentration dependent manner; Compared with the model group, the expression of Mvh, Oct4, SOD2, Nrf2 and Bcl-2 protein in ovarian tissue of mice in 20 mg/kg RES group and 40 mg/kg RES group increased, while the expression of Bax decreased (P<0.01).

　　Conclusion Resveratrol has protective effect on mice with premature ovarian failure, and its mechanism may be related to inhibition of ovarian cell apoptosis and activation of Wnt/ β- Catenin pathway promotes the recovery of ovarian function.