动物造模 Z-bio 2022-12-26 265

　　Objective To study the effect of compound microecological preparation on intestinal mucosal immune function in mice.

　　Methods Eighty 28 day old BALB/C mice were selected by gavage with low, medium and high doses (2 5. 5, 10 g/kg body weight), blood samples and jejunum tissues were taken on the 14th and 28th days, and immune organs were taken for immune organ index measurement. The content of lysozyme in blood was detected by turbidimetry, and the immune cytokines (IL-4, IL-10, IFN) in mouse jejunum mucosa were detected by ELISA- γ) And SIgA content; The expression of immune cytokines mRNA in jejunum mucosa of mice was detected by fluorescent quantitative PCR.

　　Results (1) The contents of immune cytokines and SIgA and the expression of immune cytokines mRNA in intestinal mucosa of mice were increased by different doses of complex microecological agents. On the 14th day, the immune cytokines IL-4, IL-10 and IFN in jejunum mucosa of mice in medium dose group- γ And SIgA content and mRNA expression level were significantly higher than those in the control group (P<0 01)。 On the 28th day, the content of IL-4 in jejunum mucosa of low dose group and middle dose group was significantly increased (P<0 01); Medium dose group IL-10, IFN- γ And SIgA content and mRNA expression level were significantly higher than those in the control group (P<0 01); IL-4, IL-10 and IFN in medium and high dose groups- γ The mRNA expression levels of the two groups were higher than those of the control group (P<0 05 or P<0 the="" expression="" levels="" of="" il-4="" and="" il-10="" mrna="" in="" low-dose="" group="" were="" not="" significantly="" different="" from="" those="" control="" p="">0 05)。 (2) On the 14th day, the immune organ index of the medium dose group was significantly higher than that of the control group (P<0 05), on the 28th day, the immune organ index of each experimental group was significantly higher than that of the control group (P<0 05)。 (3) The content of lysozyme in each experimental group was significantly higher than that in the control group (P<0 05)。

　　Conclusion Compound microecological preparation can promote the growth of mice, increase the index of immune organs, increase the content of lysozyme in blood and the immune function of intestinal mucosa, and reduce the occurrence of intestinal infectious diseases.