动物造模,坏死性小肠结肠炎 Z-bio 2022-12-26 323

　　Objective To analyze the protective effect of amygdalin on necrotizing enterocolitis in neonatal rats.

　　Methods Sixty healthy newborn SD rats born on the 7th day were randomly divided into control group, model group, low-dose amygdalin group, middle dose amygdalin group, high-dose amygdalin group and sulfasalazine group, with 10 rats in each group. Except the control group, the rats in other groups were used to establish NEC model by hypoxia cold stress combined with invasive feeding of formula milk. At the same time, 20, 40 and 80 mg/kg amygdalin were given to low, medium and high dose groups respectively, and 300 mg/kg sulfasalazine was given to sulfasalazine group for 5 consecutive days. 12 hours after the last intervention, the weight changes of rats were recorded, and the ileocecal tissues of small intestine were taken for HE staining, and the ileocecal intestinal tissue injury score was performed; The apoptosis rate was detected by TUNEL staining; Detection of serum inflammatory factor: tumor necrosis factor (TNF) by enzyme-linked immunosorbent assay- α）、 Interleukin-6 (II-6), Interleukin-1 β （Ⅱ-1 β）、 Levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH Px). The expression levels of NOD like receptor protein 3 (NLRP3), apoptosis related spot like protein (ASC) and caspase-1 protein were detected by Western blot.

　　Results Compared with the control group, the weight of rats in the model group decreased significantly, the intestinal tissue injury score, the apoptosis rate of intestinal tissue cells, TNF- α、 IL-6、IⅡL-1 β、 The levels of SOD, MDA, GSH Px and the expression levels of NLRP3, ASC, Caspase-1 protein were significantly increased (P<0.05); Compared with the model group, the weight of rats in the middle dose group, the high dose group and the sulfasalazine group increased significantly, the intestinal tissue damage score, the apoptosis rate of intestinal tissue cells decreased significantly, and TNF- α、 I-6、IL-1 β、 The levels of SOD, MDA, GSH Px, NLRP3, ASC, Caspase-1 protein expression decreased significantly (P<0.05).

　　Conclusion Amygdalin has a certain protective effect on necrotizing enterocolitis in neonatal rats. It can effectively reduce intestinal tissue damage, reduce the body's inflammatory response, oxidative stress response, and reduce the apoptosis rate of intestinal tissue cells. However, the specific reaction mechanism is not clear enough, which needs further clinical exploration.