动物造模,帕金森病 Z-bio 2022-12-26 365

　　Objective to investigate the neuroprotective effect of dendrobium nobile extract on parkinson's disease model induced by 1-methylv-4-phenyl-1,2,3,6-tetrahydropyridine in mice.

　　Methods The mice were randomly divided into control group and model group, methobal group (positive drug group) and DNL (100, 200, 300 mg/kg) group. The subacute PD mice model was established by intraperitoneal injection of (i.p) MPTP (30 mg/(kg · d), 7 days). Gait analysis experiment, pole climbing experiment and suspension experiment were carried out to evaluate the effects of DNL on the abnormal movement of PD mice; Detection of tumor necrosis factor in striatum and cortex of mice in each group by enzyme-linked immunosorbent assay- α、 Interleukin-6 and Interleukin-1 β Changes in expression; Western blot was used to detect the expression of caspase-3 and caspase-9 in striatum of mice in each group.

　　Results Compared with the control group, the gait indexes of the model group had significant changes (P<0.05); Compared with the model group, the left posterior, right anterior and right posterior propulsive index, left posterior, right anterior and right posterior supporting phase in the medoba group and DNL (100, 200, 300 mg/kg) group were significantly improved (P<0.05); Compared with the control group, the upper half and total time of climbing pole in the model group increased (P<0.01, P<0.001), while="" the="" time="" of="" turning="" around="" and="" lower="" half="" had="" no="" significant="" change="" p="">0.05); Compared with the model group, DNL (300 mg/kg) group can improve the upper half of the time (P<0.05), 200="" metoprolol="" and="" dnl="" p="">0.05); Compared with the control group, the suspension score of the model group decreased (P<0.05). Compared with the model group, DNL (300 mg/kg) could increase the suspension score (P<0.05). 200="" medoba="" and="" dnl="" p="">0.05); Compared with control group, TNF in striatum of model group mice- α、 IL-1B. Ⅱ L-6 content increased (P<0.05), caspase-3 and caspase-9 protein expression levels increased significantly (P<0.001); Compared with the model group, the indexes of inflammatory factors in medoba and DNL (300 mg/kg) groups decreased (P<0.05), and="" there="" was="" no="" difference="" in="" the="" content="" of="" cortex="" each="" group="" p="">0.05); The expression levels of caspase-3 and caspase-9 protein decreased (P<0.05).

　　Conclusion DNL can improve the behavior changes of MPTP induced PD mice, reduce the inflammatory reaction in brain tissue, and inhibit the apoptosis of nerve cells. It has a good potential in the research and development of drugs and functional food for treating PD.