动物造模,溃疡性结肠炎 Z-bio 2022-12-27 244

　　Objective To observe the effect of mussel mucin (MAP) on intestinal mucosa of rats with ulcerative colitis, and to explore its mechanism from the perspective of adhesion and antioxidation.

　　Methods Thirty two SPF SD rats were randomly divided into normal group, model group, mesalazine group and mussel mucin group (0 6 mg/kg), 8 in each group. The normal group was given ordinary drinking water, and the other groups were given 5% dextran sulfate (DSS) free water for 7 days to prepare the model of ulcerative colitis. They were administered daily 24 hours after the model was established. The daily disease activity index (DAI) and body weight changes of rats in each group were observed and recorded. Seven days after the drug was administered, the anatomical materials were taken, the length, weight, and intestinal weight ratio of rats in each group were recorded, and the general morphology (CMDI) and histopathology of colorectal mucosa were observed. The intestinal mucosa of rats was taken for in vitro test, and the effect of MAP on the adhesion and antioxidation of intestinal mucosa of rats was observed by nitro tetrazolium chloride blue (NBT) staining.

　　Results Compared with the normal group, the DAI score of the model group increased significantly from the fifth day of the experiment (P<0 05), weight loss (P<0 05), congestion, edema and ulcer formation of intestinal mucosa can be seen, as well as swelling and deformation of colonic recess, and a large number of inflammatory cells infiltrate submucosal tissue; Compared with the model group, the body weight and colorectal length of rats in the mesalazine group and mussel mucin group increased (P<0 05), DAI score, intestinal weight ratio, CMDI score and histopathological score decreased (P<0 05)。 In the NBT experiment, the rectal mucosa of rats appeared blue staining, and the degree of blue staining was further deepened with time.

　　Conclusion MAP can obviously improve the symptoms of ulcerative colitis model rats and repair the damage of intestinal mucosal barrier through its good adhesive and antioxidant effects on intestinal mucosa, which can provide a reliable experimental basis for MAP to treat ulcerative colitis.