动物造模 Z-bio 2022-12-29 247

　　Objective To observe the effect of Qingre Runzao oral liquid on the number of type Ⅱ innate lymphocytes (ILC2s) and their upstream and downstream cytokines in asthmatic mice sensitized by ovalbumin (OVA).

　　Methods BALB/c mice (female) were divided into four groups: blank control group, model control group, positive control group (dexamethasone group, 1 mg/(kg · d)), low and high dose (4.32, 8.64 g/(kg · d)) groups. OVA was used as sensitizer to induce asthma model in mice, and HE staining was used to observe the pathological changes of lung tissue in mice; The levels of cytokines Eotaxin, IL-25, IL-33, TSLP, IL-4, IL-5 and IL-6 in BALF were measured by ELISA; Immunohistochemistry was used to detect the expression of cytokines IL-33 and IL-5 upstream and downstream of ILC2s in lung tissue; The quantity and production of ILC2s in lung tissue were detected by flow cytometry and immunofluorescence staining.

　　Results Compared with the blank control group, the model control group mice showed pathological changes of inflammatory cell infiltration in lung tissue and bronchial wall thickening, the content of cytokines Eotaxin, IL-25, IL-33, TSLP, IL-4, IL-5 and IL-6 in the upstream and downstream of ILC2s in BALF increased (P<0.01), the expression of cytokines IL-33 and IL-5 in the upstream and downstream of ILC2s in lung tissue increased (P<0.01), and the number and production of ILC2s increased (P<0.01). Compared with the model control group, Qingre Runzao oral liquid at low and high doses significantly improved the pathological damage of lung tissues in asthmatic mice, decreased the expression of upstream and downstream cytokines of ILC2s in BALF (P<0.01), decreased the expression of upstream and downstream cytokines of ILC2s in lung tissues (P<0.05, P<0.01), and decreased the number and production of ILC2s (P<0.01).

　　Conclusion Qingre Runzao oral liquid can reduce the number of ILC2s and its upstream and downstream cytokines in OVA induced asthmatic mice, and down regulate the expression of IL-33 and IL-5 in lung tissue, thus playing a role in preventing and treating asthma.