动物实验,盐酸椒苯酮胺 z-bo 2020-08-22 567

　　OBJECTIVE: Guinea pig cochlear autophagy-related proteins beclin1 and LC3, Na + -K + -2Cl- complex transporter (NKCC1) mRNA, endothelin (ET) expression, and cochlear gentamicin (GM) damage and pifectin The relationship between hydrochloride. Observe (PPTA) the protective effect and mechanism of injury.

　　Method: 60 guinea pigs were randomly divided into control group, model group, simultaneous treatment group, post-model control group and treatment group. The control group received NS + artificial lymph, the model group received GM + artificial lymph, and the treatment group received GM + PPTA (both for 7 consecutive days). After modeling, the control group and post-treatment group were infused with GM for 7 consecutive days. Artificial lymph and PPTA were injected for 7 consecutive days. In all guinea pigs, the ear capsules were surgically placed, NS and GM (160 mg? kg-1?d-1) were injected intraperitoneally, and the ear capsules were injected into artificial lymph and PPTA. After treatment of each group of guinea pigs, the hearing was analyzed by ABR test, and the expression of beclin1 and LC3, NKCC1 mRNA and ET-1 were detected.

　　Results: After modeling, compared with the control group, there was no significant difference between the ABR results (P\→0.05), but they were all significantly higher than the other three groups (P\u003c0 .05). In the late treatment group (P\u003c0.05), the expression of LC3II and Beclin1 in the model group was significantly higher than the other four groups. The expression of LC3II and Beclin1 in the model 3 late treatment group was the control group. The expression of NKCC1 mRNA in the model group was higher than the other 4 groups. 4 After modeling, the expression of NKCC1 mRNA in the treated group was significantly lower than that in the control group (P\u003c0). 05). The expression of ET-1 in each part of the model group was significantly higher than the other 4 groups. The expression of ET-1 in each part of the control group was lower than the other 4 groups. After modeling, the expression of ET-1 in each part of the treatment group was lower than that of the control group.

　　Conclusion: PPTA inhibits the autophagy of cochlear cells, the expression of NKCC1 and ET-1, and protects the cochlea from gentamicin damage; PPTA is more effective for cochlear damage caused by gentamicin, while GM is more effective and suggests that Hearing cells cause irreversible damage.