动物实验 z-bo 2020-08-22 542

　　Purpose: DEHP exposure has in vivo effects on mouse placental growth and development, maternal-fetal interface uNK cell number and angiogenesis through exposure to bis(2-ethylhexyl) phthalate during pregnancy. Was observed.

　　Method: Give different doses of DEHP125, 250, 500mg by gavage from the first day of pregnancy? kg-1? d-1. Collect samples of uterus and placenta and weigh them on the 13th day of pregnancy. Be tested. , HE staining and immunohistochemical methods to observe the effect of maternal-fetal interface on mouse placenta growth and development, uNK cell number and angiogenesis.

　　Result: (1) Low-dose DEHP125 250mg? kg-1? Compared with the control group, d-1 had no significant effect on the number of embryo implantation (P \u003c0.05). However, 500mg in the high-dose group? kg-1? d-1DEHP significantly reduced the number of embryo implantation (P\u003c0.05). In addition, exposure to DEHP can significantly increase the rate of fetal miscarriage. (2) Compared with the control group (0.0786±0.0143g), the placental weight of DEHP was 125, 250, 500 mg·kg-1·d-1 (0.0637±0.0133, 0.0587±0.0176, 0.0524±). 0.0183g; P\u003c0.01), the total placental area and trophoblast area in the sponge layer were significantly reduced; the number was significantly reduced, and the blood vessels collapsed and closed; (4) Compared with the control group, DEHP exposure was dose-dependently Inhibit the number of uNK cells (105.1±14.2/HP) (respectively: 83.2). ±10.3, 60.7±12.4), 50.4±14.5/HP; P\u003c0.01).

　　Conclusion: Exposure to DEHP during pregnancy may significantly inhibit the growth and development of the placenta, which may affect the formation of blood vessels and the number of uNK cells at the border between mother and infant.