动物造模 Z-bio 2023-02-08 289

　　Objective To study the effect of resveratrol on α- Effects of Synuclein A53T transgenic mice on motor dysfunction and peripheral immunity.

　　Methods Eight-month-old A53T transgenic mice were randomly divided into four groups: control group (WT), control treatment group (WT+RES), model group (PD) and model treatment group (PD+RES). The treatment group was given RES every 3 days for 3 months; The control group and model group were given the same amount of normal saline. The movement level of mice in each group was tested by climbing rod test, rotating rod test, forefoot grip test and limb holding test. T lymphocyte subsets of mice were detected by flow cytometry, and interleukin 6 (IL 6) and tumor necrosis factor in mouse serum were detected by enzyme linked immunosorbent assay (ELISA)- α ( TNF α)、 Interleukin-18 (IL-18) and transforming growth factor- β (TGF- β) Level.

　　Results Compared with the control group, the motor function score of the model group was lower; Compared with the model group, the motor function evaluation of the model treatment group was significantly improved. The flow cytometry results showed that compared with the control group, the proportion of peripheral T cells, CD4+T cells and the ratio of CD4+/CD8+in the model group decreased, while the proportion of CD8+T cells was not statistically significant. Compared with the model group, the proportion of T lymphocytes and CD4+T cells in the model treatment group increased significantly, with statistical significance. The ratio of CD4+/CD8+also increased, but the difference was not statistically significant. The results of ELISA in peripheral blood serum showed that compared with the control group, IL-6 and IL-18 in the model group increased, and TGF- β Lowering, TNF- α There was no statistical difference in concentration. Compared with the model group, the concentrations of IL-6 and IL-18 in the model treatment group decreased significantly, and TGF- β The result was not statistically significant.

　　Conclusion RES can significantly improve the motor dysfunction of A53T transgenic mice by regulating peripheral immunity and reducing neuroinflammatory reaction.