动物造模,肺癌异位皮下移植瘤 Z-bio 2023-02-08 564

　　Objective To investigate the effect of different concentrations of Matrigel on heterotopic subcutaneous transplantation of Lewis lung cancer in mice.

　　Methods 24 C57BL/6J Nifdc mice were randomly divided into lung cancer group, 50% matrix glue group, 75% matrix glue group, and 100% matrix glue group, with 6 mice in each group. Lewis lung cancer cells (LL/2) were mixed with 0%, 50%, 75%, and 100% Matrigel respectively according to the volume of 1 ∶ 1, and subcutaneously injected into the armpit of the right forearm of mice. The weight, diet and water consumption of mice in each group were measured daily; Observe and compare the tumor formation time, tumor formation rate and tumor size of each component, measure the long and short diameter of tumor every day after tumor formation, and calculate the change of tumor volume; The animals were killed on the 15th day of tumor inoculation, the tumor tissue was completely stripped and weighed, and the pathological changes of tumor tissue were detected by HE staining.

　　Results Compared with the lung cancer group, the weight of mice in the 75% matrix glue group increased significantly from the second day of modeling (P<0. 05) 05 or P<0 01); The weight of the mice in the 100% matrix glue group increased significantly from the 10th day of modeling (P<0 05 or P<0 01)。 The average diet of mice in each group decreased after 8 days of modeling, and the water consumption showed an overall upward trend. The tumorigenic rate of mice in each group was 100%. The mice in the matrix glue group had earlier tumorigenic time, faster tumor growth rate and larger tumor than those in the lung cancer group. In the 75% matrix glue group, the tumor volume increased the fastest and the tumor weight was the largest (1358 88 ± 388. 14) Mg, compared with the tumor group, there was a significant difference (P<0 05); In the 100% matrix glue group, the tumor volume increased next, and the tumor weight was (1142 37 ± 423. 08) mg, significantly different from the tumor group (P<0 05); In the 50% matrix glue group, the tumor volume grew the slowest and the tumor weight was the smallest (808 83 ± 393. 41) mg。 The HE staining results showed that compared with the lung cancer group, the tumor cells in the group added with matrix glue grew vigorously, with clear outline, larger nuclear volume, and significantly increased vascular components in the tumor tissue stroma.

　　Conclusion Matrix glue can stabilize the tumorigenesis rate, accelerate the growth of tumor, and increase the tumorigenesis volume. At 75% concentration of matrix glue, tumor growth is the fastest, tumor volume is the largest, and tumor uniformity is good.