动物造模 Z-bio 2023-02-08 541

　　Objective To study the growth and metabolism of high-sugar and high-fat foods in inbred BALB/c and C57BL/6J mice, and explore the differential effects of HSHF foods on their growth and metabolism based on different strains and sexes of mice.

　　Methods After weaning on the 25th day, the offspring of two strains were fed by sex. One group was fed with maintenance feed (standard food), and the other group was fed with high sugar and high fat (HSHF) feed. Weigh the body weight once every two weeks until the end of the experiment for 24 weeks, and take samples after euthanasia. The growth curve, blood glucose, blood lipid level, organ index (spleen, liver, lung) and other parameters and organ histopathology were analyzed.

　　Results The growth curve, blood glucose, serum low density lipoprotein and triglyceride levels of animals were directly related to their strains; Animal sex has an important influence on these parameters. HSHF food significantly changed the growth curve of females, but had no significant effect on males; The metabolic parameters were improved, and the effect on serum lipids was strain or sex specific. The degree of liver fat change and the change of spleen index were related to the strain and sex; No histopathological changes were found in the lung.

　　Conclusion The changes of growth and metabolism of mice caused by HSHF food have significant correlation with the strain and sex of animals, and the correlation of sex is more through strain.