动物造模,肾中毒 Z-bio 2023-02-08 534

　　Objective To explore the mechanism of detoxification and protection of grub peptide extract on mice by establishing a lead acetate induced nephrotoxicity model in order to provide experimental basis for the prevention and treatment of lead induced nephrosis.

　　Methods Mice were randomly divided into control group, model group, positive drug group and grub peptide group with different doses (80, 160, 320 mg/kg). Except for the control group, mice in all groups were intraperitoneally injected with 20 mg/kg lead acetate for 15 days. At this time, the mice in the control group and model group were fed with normal saline at the same time, while the mice in the positive drug group were fed with DMSA 70 mg/kg suspension, and the mice in the grub peptide group were fed with different doses of grub peptide extract once a day for 15 consecutive days. The renal tissue status was observed by HE staining microscope; The indexes of renal function (BUN, Cr), the level of antioxidant enzymes (SOD, GSH-Px) and the content of peroxide (MDA) in renal tissue were measured; RT-PCR and Western blot were used to detect and analyze the gene and protein expression levels of phase II detoxification enzyme (NQO1), antioxidant enzyme (HO-1) and signal molecule (Nrf2).

　　Results Compared with the model group, the grub peptide group gained weight but was lower than the control group, the renal tissue morphology was significantly improved, and the serum BUN and Cr levels were significantly decreased (P<0. 05) 05), the levels of antioxidant enzymes (SOD, GSH-Px) in kidney tissue were significantly increased (P<0 05), the content of MDA was significantly decreased, and the expression levels of phase II detoxification enzyme gene (NQO1), antioxidant enzyme (HO-1) and signal molecule (Nrf2) mRNA and protein were significantly increased (P<0. 05) 01)。

　　Conclusion The grub peptide extract can activate Nrf2-ARE signal pathway, enhance the antioxidant function of lead poisoning mice and increase the expression of detoxification enzyme gene to play its detoxification and protection role.