动物造模,妊娠高血压 Z-bio 2023-02-08 568

　　Objective To investigate the effect of matrine on endothelial injury and tyrosine protein kinase 2/signal transducer and activator of transcription 3/cytokine signal transcription inhibitor 1 (JAK2/STAT3/SOSC1) signal pathway in rats with pregnancy induced hypertension (PIH).

　　Methods 60 pregnant rats were randomly divided into normal control group, model control group, low-dose matrine group, high-dose matrine group and magnesium sulfate group, with 12 rats in each group. PIH rat models were established by intragastric administration of 50 mg/kg nitrosyl L-arginine methyl ester on the 12th day of pregnancy in pregnant rats other than the normal control group. On the 16th day of pregnancy, the low and high dose matrine groups were gavaged with 50 and 100 mg/kg matrine, the magnesium sulfate group was gavaged with 100 mg/kg magnesium sulfate, the normal control group and the model control group were gavaged with the same volume of normal saline. The tail artery blood pressure and 24-hour urine protein content of pregnant rats in each group were measured by non-invasive sphygmomanometer and Coomassie brilliant blue method respectively on the 16th day (before administration), the 17th day and the 21st day of pregnancy; Detection of superoxide dismutase (SOD), malondialdehyde (MDA) and tumor necrosis factor in serum with enzyme-linked immunosorbent assay kit α (TNF- α) 、 Interleukin (IL) - 6, IL-10, endothelin (ET), thromboxane B2 (TXB2), nitric oxide (NO), 6-ketoprostaglandin F1 α (6-keto-PGF1 α) Level; The expression of JAK2, p-JAK2, STAT3, p-STAT3 and SOSC1 protein in rat placenta was detected by Western blot.

　　Results On the 17th and 21st day of pregnancy, the blood pressure and 24-hour urine protein content in the model control group were significantly higher than those in the normal control group (P<0.01) 05), the blood pressure and 24-hour urine protein content of pregnant rats in low and high dose matrine group were significantly lower than those in model control group (P<0.05) 05) 。 Compared with the normal control group, SOD, IL-10, NO and 6-keto-PGF1 in the serum of rats in the model control group α Lower level, MDA, TNF- α、 The levels of IL-6, ET, TXB2 and p-JAK2/JAK2, p-STAT3/STAT3, and SOSC1 protein in placenta tissue increased (P<0.01) 05) ; Compared with model control group, SOD, IL-10, NO and 6-keto-PGF1 in low and high dose matrine group α Increase in sequence, MDA, TNF- α、 The levels of IL-6, ET, TXB2 and p-JAK2/JAK2, p-STAT3/STAT3, and SOSC1 protein in placenta tissue decreased in turn (P<0.05) p="">0. 05) 。

　　Results On the 17th and 21st day of pregnancy, the blood pressure and 24-hour urine protein content in the model control group were significantly higher than those in the normal control group (P0 05) 。 Conclusion Matrine can reduce blood pressure, urinary protein content, inflammatory reaction and oxidative stress level in PIH rats, inhibit JAK2, STAT3 phosphorylation and SOSC1 protein expression, and alleviate endothelial injury in PIH rats.