动物造模,缺氧性脑损伤修复 Z-bio 2023-02-09 658

　　Objective To explore the effect of resveratrol through HIF-1 α/ The mechanism of BNIP3-mediated autophagy signal pathway promoting the repair of hypoxic brain injury in mice.

　　Methods The intermittent hypoxia model of mice was established by CQY-1 small animal hypoxia detection system. The changes of hypoxia tolerance time, respiratory rate and heart rate were measured. Histopathological staining was used to detect the morphological changes of rat brain. Detection of HIF-1 in mouse brain tissue by Western blot α、 Protein expression level of P53, BNIP3, Beclin 1, LC3 and P62.

　　Results Compared with the hypoxic group, resveratrol could prolong the hypoxia tolerance time of mice, increase the respiratory rate of hypoxic mice, reduce the heart rate of hypoxic mice, alleviate the brain tissue damage in the hippocampus, and down-regulate HIF-1 α、 P53, Beclin 1, LC3 protein expression, up-regulate P62 protein expression.

　　Conclusion Resveratrol down-regulates HIF-1 α/ The autophagy signal pathway mediated by BNIP3 can promote the repair of hypoxic brain injury in mice.